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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Urokinase plasminogen activator, uPA
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Urokinase plasminogen activator, uPA ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
uPA,ATF; BDPLT5; QPD; URK; u-PA; U-plasminogen activator; plasminogen activator urinary; plasminogen activator urokinase, Urokinase-type plasminogen activator
Search name
Mouse uPA ELISA KIT ,Mouse ATF ELISA KIT ,Mouse BDPLT5 ELISA KIT ,Mouse QPD ELISA KIT ,Mouse URK ELISA KIT ,Mouse u-PA ELISA KIT ,Mouse U-plasminogen activator ELISA KIT ,Mouse plasminogen activator urinary ELISA KIT ,Mouse plasminogen activator urokinase ELISA KIT ,Mouse Urokinase-type plasminogen activator ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse urokinase-type plasminogen activator,uPA concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to uPA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for uPA and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain uPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of uPA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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attachments. One such enzyme, the serine protease urokinase-type plasminogen activator (uPA), converts enzymatically inactive plasminogen into the widely acting protease plasmin, which degrades several extracellular matrix components and also activates
is almost catalytic when compared to antisense silencing. Of these targets, the uPAR-uPA system and MMPs holds great promise. Targeting uPA/uPAR may provide additive or synergistic treatment benefits if used in combination with conventional therapeutics
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