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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Protein FAM40B, Fam40b
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Protein FAM40B, FAM40B ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Fam40b,STRIP2,protein FAM40B; FAM40B; FAR11B; FAR11 factor arrest 11 homolog B; family with sequence similarity 40, member B; homolog of yeast FAR11 protein 2; striatin-interacting protein 2; striatin interacting protein 2
Search name
Mouse Fam40b ELISA KIT ,Mouse STRIP2 ELISA KIT ,Mouse protein FAM40B ELISA KIT ,Mouse FAR11B ELISA KIT ,Mouse FAR11 factor arrest 11 homolog B ELISA KIT ,Mouse family with sequence similarity 40 ELISA KIT ,Mouse member B ELISA KIT ,Mouse homolog of yeast FAR11 protein 2 ELISA KIT ,Mouse striatin-interacting protein 2 ELISA KIT ,Mouse striatin interacting protein 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Protein FAM40B, FAM40B concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein FAM40B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Protein FAM40B and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Protein FAM40B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Protein FAM40B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product
Delivery time 4-6month
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文献和实验, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
The indirect ELISA is used primarily to determine the strength and/or amount of antibody response in a sample, whether it is from the serum of an immunized animal or the cell supernatant from growing hybridoma clones. Procedure 1. All incubations
The indirect ELISA is used primarily to determine the strength and/or amount of antibody response in a sample, whether it is from the serum of an immunized animal or the cell supernatant from growing hybridoma clones. Procedure
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