- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELA-E2068m
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Uncoupling Protein 3含量
- 适应物种：
小鼠
- 标记物：
Uncoupling Protein 3
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Uncoupling Protein 3 ELISA试剂盒
Product Name: Mouse Uncoupling Protein 3 ELISA试剂盒
Product Name: Mouse Uncoupling Protein 3 ELISA试剂盒
Gene Name: Mouse Uncoupling Protein 3
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Uncoupling Protein 3
Alternative Name: Mouse Uncoupling Protein 3
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Uncoupling Protein 3 ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Uncoupling Protein 3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Uncoupling Protein 3 ELISA试剂盒 has been pre-coated with an Mouse Uncoupling Protein 3 antibody specific to Mouse Uncoupling Protein 3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Uncoupling Protein 3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Uncoupling Protein 3 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Uncoupling Protein 3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Detection of Uncoupling Protein-2 (UCP2) As a Mitochondrial Modulator of Apoptosis
There is an increasing evidence that uncoupling protein-2 (UCP2), a recently identified molecular sensor and suppressor of mitochondrial reactive oxygen species (ROS), plays an important role in �regulating apoptosis in different cell systems
Immortalised cultured cells are powerful tools to assess the function of ectopically expressed proteins. However, it must be ensured that the protein of interest is functional in the host system and display native behaviour. In particular
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
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