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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
F-box only protein 28, Fbxo28
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse F- box only protein 28, Fbxo28 ELISA KIT
Product Name:Mouse F- box only protein 28, Fbxo28 ELISA KIT
Packing:96T
Catalog No.:ELI-32508m
Gene Name:Mouse Fbxo28
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Fbxo28
Alternative Name:Mouse F-box only protein 28, Fbxo28
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse F- box only protein 28, Fbxo28 ELISA KIT allows for the in vitro quantitative determination of Mouse Fbxo28 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse F- box only protein 28, Fbxo28 ELISA KIT has been pre-coated with an Mouse F-box only protein 28, Fbxo28 antibody specific to Mouse Fbxo28 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Fbxo28 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Fbxo28 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Fbxo28 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Image Figure 2.12.5 Alignment of Grb2 protein sequences. Alignment of human, mouse, rat, worm, fly, and mosquito Grb2 sequences generated
Immunohistochemistry: Fluorescence Protocol 2.0
by centrifugation (400 x g for 5 minutes). Incubate the cells for 30 minutes at room temperature with 50 µL of a biotinylated secondary antibody (either biotin-donkey anti-goat IgG Fab2 diluted 1:700 or biotin-goat anti-mouse IgG1 or IgG2A or IgG2B diluted
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
sapiens F-box only protein 7 (FBXO7), mRNA 865 NM_020360 Homo sapiens phospholipid scramblase 3 (PLSCR3), mRNA 3645 NM_014891 Homo sapiens PDGFA associated protein 1 (PDAP1), mRNA 1045 NM_005745 Homo sapiens accessory protein BAP31 (DXS1357E
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