Keratin-associated protein 15-
1, Krtap15-1 ELISA KIT/ Mouse Keratin-associated protein 15-1, Krtap15-1 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-31821m
- 2025年07月08日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Keratin-associated protein 15-1, Krtap15-1
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Keratin- associated protein 15- 1, Krtap15-1 ELISA KIT
Product Name:Mouse Keratin- associated protein 15- 1, Krtap15-1 ELISA KIT
Packing:96T
Catalog No.:ELI-31821m
Gene Name:Mouse Krtap15-1
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Krtap15-1
Alternative Name:Mouse Keratin-associated protein 15-1, Krtap15-1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Keratin- associated protein 15- 1, Krtap15-1 ELISA KIT allows for the in vitro quantitative determination of Mouse Krtap15-1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Keratin- associated protein 15- 1, Krtap15-1 ELISA KIT has been pre-coated with an Mouse Keratin-associated protein 15-1, Krtap15-1 antibody specific to Mouse Krtap15-1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Krtap15-1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Krtap15-1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Krtap15-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Transient Transfections and Heterokaryons as Tools for the Analysis of Keratin IF Dynamics
(neutral-basic) keratins, whose expression is carefully controlled in a cell type- and differentiation-specific manner (1 –3 ). In addition, many epithelial cells in culture also induce the synthesis of the IF protein vimentin. In contrast
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
stock solution Nutrient-rich fly medium Phosphate-buffered saline (PBS; pH 7.5) Poly-L-lysine solution (0.1% [w/v] in H2 O; Sigma P8920) Sodium phosphate buffer (10 mM, pH 6.8) Triton X-100 VECTASTAIN Elite ABC Kit
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
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