Dihydrofolate reductase, Dhfr
ELISA KIT/ Mouse Dihydrofolate reductase, Dhfr ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-32431m
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Dihydrofolate reductase, Dhfr
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Dihydrofolate reductase, Dhfr ELISA KIT
Product Name:Mouse Dihydrofolate reductase, Dhfr ELISA KIT
Packing:96T
Catalog No.:ELI-32431m
Gene Name:Mouse Dhfr
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Dhfr
Alternative Name:Mouse Dihydrofolate reductase, Dhfr
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Dihydrofolate reductase, Dhfr ELISA KIT allows for the in vitro quantitative determination of Mouse Dhfr concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Dihydrofolate reductase, Dhfr ELISA KIT has been pre-coated with an Mouse Dihydrofolate reductase, Dhfr antibody specific to Mouse Dhfr .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Dhfr and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Dhfr , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Dhfr in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Screening Libraries for Improved Solubility: Using E. coli Dihydrofolate Reductase as a Reporter
. In this article, we describe such a method. The method relies on the fact that increasing the expression of dihydrofolate reductase (DHFR) makes Escherichia coli resistant to Trimethoprim (TMP). Proteins fused to DHFR will produce chimeras
Using the DHFR Heat-Inducible Degron for Protein Inactivation in Schizosaccharomyces pombe
Inactivating a specific protein in vivo can yield important information about its function. One strategy previously developed inSaccharomyces cerevisiae by the Varshavsky group involves fusing a degron, derived from mouse dihydrofolate
Cytogenetic Characterization of Recombinant Cells
; therefore a selectable system is desirable to discriminate cells that have integrated the foreign DNA. Chinese hamster ovary (CHO) cells are most commonly used because of the availability of both a dihydrofolate reductase (DHFR)-deficient mutant cell line (CHO-DUKX
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