Elongation factor 2, Eef2 ELIS

Mouse Elongation factor 2, EEF2 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Elongation factor 2,EF-2, EEF2,eukaryotic translation elongation factor 2; elongation factor 2; EEF-2; EF-2; EF2; polypeptidyl-tRNA translocase

Search name

Mouse Elongation factor 2 ELISA KIT ,Mouse EF-2 ELISA KIT ,Mouse  EEF2 ELISA KIT ,Mouse eukaryotic translation elongation factor 2 ELISA KIT ,Mouse elongation factor 2 ELISA KIT ,Mouse EEF-2 ELISA KIT ,Mouse EF-2 ELISA KIT ,Mouse EF2 ELISA KIT ,Mouse polypeptidyl-tRNA translocase ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of mouse elongation factor 2,EF-2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to EF-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for EF-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain EF-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of EF-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

PDF Manual

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