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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Elongation factor 2, Eef2
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Elongation factor 2, EEF2 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Elongation factor 2,EF-2, EEF2,eukaryotic translation elongation factor 2; elongation factor 2; EEF-2; EF-2; EF2; polypeptidyl-tRNA translocase
Search name
Mouse Elongation factor 2 ELISA KIT ,Mouse EF-2 ELISA KIT ,Mouse EEF2 ELISA KIT ,Mouse eukaryotic translation elongation factor 2 ELISA KIT ,Mouse elongation factor 2 ELISA KIT ,Mouse EEF-2 ELISA KIT ,Mouse EF-2 ELISA KIT ,Mouse EF2 ELISA KIT ,Mouse polypeptidyl-tRNA translocase ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse elongation factor 2,EF-2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to EF-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for EF-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain EF-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of EF-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual
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