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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Growth含量
- 适应物种:
小鼠
- 标记物:
Growth
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
differentiation factor 8, GDF8 ELISA Kit
Product Name: differentiation factor 8, GDF8 ELISA Kit
Product Name: differentiation factor 8, GDF8 ELISA Kit
Gene Name: differentiation factor 8, GDF8 ELISA Kit
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: differentiation factor 8, GDF8 ELISA Kit
Alternative Name: differentiation factor 8, GDF8 ELISA Kit
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: differentiation factor 8, GDF8 ELISA Kit allows for the in vitro quantitative determination of differentiation factor 8, GDF8 ELISA Kit concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in differentiation factor 8, GDF8 ELISA Kit has been pre-coated with an differentiation factor 8, GDF8 ELISA Kit antibody specific to differentiation factor 8, GDF8 ELISA Kit .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for differentiation factor 8, GDF8 ELISA Kit and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain differentiation factor 8, GDF8 ELISA Kit, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of differentiation factor 8, GDF8 ELISA Kit in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous System
from the developing mouse and adult cortex. RELATED INFORMATION This protocol was adapted from the method by Polleux and Ghosh (2002) . MATERIALS Reagents Dulbecco’s phosphate-buffered saline (PBS), 1X sterile (Sigma D8537) Enzyme
mES cell growth medium mES cell growth medium with Leukemia Inhibitory Factor (LIF) OPTI-MEM (+HEPES, +sodium bicarbonate, +glutamine) (GIBCO/Invitrogen) Sleeping Beauty transposon and transposase plasmids (available from the Center
Transgenic Mouse and Transgenic Rat Outline
such as lacZ or a transcription factor). DNA is prepared and microinjected into fertilized mouse eggs. Potentially transgenic mice or rats are born. Transgenic founders are identified, usually by a simple PCR assay, and bred to produce offspring for analysis
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