Dual specificity protein phosp
hatase 13, Dusp13 ELISA KIT/ Mouse Dual specificity protein phosphatase 13, Dusp13 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-32285m
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Dual specificity protein phosphatase 13, Dusp13
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Dual specificity protein phosphatase 13, Dusp13 ELISA KIT
Product Name:Mouse Dual specificity protein phosphatase 13, Dusp13 ELISA KIT
Packing:96T
Catalog No.:ELI-32285m
Gene Name:Mouse Dusp13
Detect Range:15.62-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Dusp13
Alternative Name:Mouse Dual specificity protein phosphatase 13, Dusp13
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Dual specificity protein phosphatase 13, Dusp13 ELISA KIT allows for the in vitro quantitative determination of Mouse Dusp13 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Dual specificity protein phosphatase 13, Dusp13 ELISA KIT has been pre-coated with an Mouse Dual specificity protein phosphatase 13, Dusp13 antibody specific to Mouse Dusp13 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Dusp13 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Dusp13 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Dusp13 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Dual- and Triple-Color Fluorospot
实验原理 ELISPOT has, due to its high sensitivity and simplicity, proven valuable for assessing antigen-specific T-cell responses, both with regard to specificity and magnitude. One limitation of the regular ELISPOT method
system, or interaction trap, is one of the most versatile methods available with which to identify and establish protein?protein interactions. The dual bait system is one adaptation of the classic approach. This system facilitates the simultaneous comparison
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
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