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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
COX assembly mitochondrial protein homolog, Cmc1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse COX assembly mitochondrial protein homolog, Cmc1 ELISA KIT
Product Name:Mouse COX assembly mitochondrial protein homolog, Cmc1 ELISA KIT
Packing:96T
Catalog No.:ELI-31946m
Gene Name:Mouse Cmc1
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Cmc1
Alternative Name:Mouse COX assembly mitochondrial protein homolog, Cmc1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse COX assembly mitochondrial protein homolog, Cmc1 ELISA KIT allows for the in vitro quantitative determination of Mouse Cmc1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse COX assembly mitochondrial protein homolog, Cmc1 ELISA KIT has been pre-coated with an Mouse COX assembly mitochondrial protein homolog, Cmc1 antibody specific to Mouse Cmc1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cmc1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cmc1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cmc1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验线粒体荧光探针大全:TMRM,Mitotracker,JC-1
(COX) structure and mitochondrial biogenesis, Molecular Probes offers subunit-specific mouse anti�OxPhos Complex IV monoclonal antibodies that have been derived from the human, bovine and yeast forms of COX. COX catalyzes the transfer of electrons
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
mitochondrial protein, mRNA 1087 NM_004255 Homo sapiens cytochrome c oxidase subunit Va (COX5A), nuclear gene encoding mitochondrial protein, mRNA 853 NM_001788 Homo sapiens CDC10 cell division cycle 10 homolog (S. cerevisiae) (CDC10), mRNA 1340 NM
Ⅳ:COX4)是定位在线粒体内膜上的膜蛋白,凋亡发生时,它保留在线粒体内,因而它是线粒体富集部分的一个非常有用的标志。 ApoAlertTMCell Fractionation Kit不用超离心,可从凋亡和非凋亡细胞中快速有效分离出高度富集的线粒体部分,再进一步通过Western杂交用细胞色素C抗体和COX4抗体标示细胞色素C和COX4的存在位置,从而判断凋亡的发生。 [next]4) 线粒体膜电位变化的检测: 在凋亡研究的早期,从形态学观测上线粒体没有明显的变化
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