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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Cytochrome P450 1A2, Cyp1a2
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Cytochrome P450 1A2, Cyp1a2 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Class 1.14.14.1,Cytochrome P450 1A2,Cytochrome P450-D,Cytochrome P-450d,CYPIA2,Cytochrome P-448, CYP1A2,cytochrome P450 1A2; CP12; P3-450; P450(PA); CYPIA2; P450 form 4; aryl hydrocarbon hydroxylase; cytochrome P(3)450; cytochrome P450 4; cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 2; cytochrome P450-P3; dioxin-inducible P3-450; flavoprotein-linked monooxygenase; microsomal monooxygenase; xenobiotic monooxygenase; cytochrome P450 family 1 subfamily A polypeptide 2
Search name
Mouse Class 1.14.14.1 ELISA KIT ,Mouse Cytochrome P450 1A2 ELISA KIT ,Mouse Cytochrome P450-D ELISA KIT ,Mouse Cytochrome P-450d ELISA KIT ,Mouse CYPIA2 ELISA KIT ,Mouse Cytochrome P-448 ELISA KIT ,Mouse CYP1A2 ELISA KIT ,Mouse cytochrome P450 1A2 ELISA KIT ,Mouse CP12 ELISA KIT ,Mouse P3-450 ELISA KIT ,Mouse P450(PA) ELISA KIT ,Mouse CYPIA2 ELISA KIT ,Mouse P450 form 4 ELISA KIT ,Mouse aryl hydrocarbon hydroxylase ELISA KIT ,Mouse cytochrome P(3)450 ELISA KIT ,Mouse cytochrome P450 4 ELISA KIT ,Mouse cytochrome P450 ELISA KIT ,Mouse subfamily I (aromatic compound-inducible) ELISA KIT ,Mouse polypeptide 2 ELISA KIT ,Mouse cytochrome P450-P3 ELISA KIT ,Mouse dioxin-inducible P3-450 ELISA KIT ,Mouse flavoprotein-linked monooxygenase ELISA KIT ,Mouse microsomal monooxygenase ELISA KIT ,Mouse xenobiotic monooxygenase ELISA KIT ,Mouse cytochrome P450 family 1 subfamily A polypeptide 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Cytochrome P450 1A2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 1A2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cytochrome P450 1A2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cytochrome P450 1A2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cytochrome P450 1A2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Cytochrome P450 Reconstitution Systems
Human liver microsomes contain multiple forms of cytochrome P450 (CYP or P450) that catalyze oxidation of a number of xenobiotic and endobiotic chemicals. Individual P450 forms have unique, but overlapping, substrate specificities
Evaluation of Cytochrome P450 Activities in Human Hepatocytes In Vitro
Major hepatic cytochrome P450 activities (CYP1A2, CYP2A6, CYP2B6, CYP2C9, CYP2C19, CYP2D6, CYP2E1, and CYP3A4) can be simultaneously examined in human hepatocytes by incubation with a cocktail of multiple specific probes. Cocktail strategy
In Vitro Identification of Cytochrome P450 Enzymes Responsible for Drug Metabolism
Metabolism catalyzed by the cytochrome P450 enzymes (CYPs) represents the most important pathway for drug metabolism and elimination in humans. Identification of the CYPs responsible for metabolism of existing and novel drugs is critical
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