Disco-interacting protein 2 ho
molog B, Dip2b ELISA KIT/ Mouse Disco-interacting protein 2 homolog B, Dip2b ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-46872m
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Disco-interacting protein 2 homolog B, Dip2b
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Disco- interacting protein 2 homolog B, Dip2b ELISA KIT
Product Name:Mouse Disco- interacting protein 2 homolog B, Dip2b ELISA KIT
Packing:96T
Catalog No.:ELI-46872m
Gene Name:Mouse Dip2b
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Dip2b
Alternative Name:Mouse Disco-interacting protein 2 homolog B, Dip2b
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Disco- interacting protein 2 homolog B, Dip2b ELISA KIT allows for the in vitro quantitative determination of Mouse Dip2b concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Disco- interacting protein 2 homolog B, Dip2b ELISA KIT has been pre-coated with an Mouse Disco-interacting protein 2 homolog B, Dip2b antibody specific to Mouse Dip2b .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Dip2b and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Dip2b , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Dip2b in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验元、胶质细胞之间的网络结构图，但脑组织中的脂质、血细胞等成分阻碍了对于神经系统和大脑结构的观察，所以透明化是十分有必要的。但传统的方法，如冷冻切片存在明显的缺点，无法观察连续的 3D 层面上的结构网络，工作量大且破坏神经组织，拍摄成像速度慢，一般至少需要数周，所以需要建立完整的大脑透明技术。BABB, DISCO, CLARITY, PACT/PARS, CUBIC 和 Scale 等在内的有效的组织清除方法的最新发展 [3-8]，可以与光片荧光显微镜结合使用对成年小鼠大脑进行快速高分辨率成像
Phage‐Based Expression Cloning to Identify Interacting Proteins
expression cloning is a simple, rapid, and powerful technique to identify interacting proteins. A protein of interest is expressed as a recombinant fusion protein and labeled with 32 P at an engineered recognition site to facilitate detection. b?gal proteins
Interaction Trap/Two‐Hybrid System to Identify Interacting Proteins
, consisting of the DNA‐binding domain of LexA fused to the probe or bait protein, shown as being unable to activate either of the two reporters. (B ) and (C ) The resulting bait strain has been additionally transformed with an activation domain (act)–fused
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