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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Cyclin-dependent kinase 12, Cdk12
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Cyclin- dependent kinase 12, Cdk12 ELISA KIT
Product Name:Mouse Cyclin- dependent kinase 12, Cdk12 ELISA KIT
Packing:96T
Catalog No.:ELI-25151m
Gene Name:Mouse Cdk12
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Cdk12
Alternative Name:Mouse Cyclin-dependent kinase 12, Cdk12
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Cyclin- dependent kinase 12, Cdk12 ELISA KIT allows for the in vitro quantitative determination of Mouse Cdk12 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Cyclin- dependent kinase 12, Cdk12 ELISA KIT has been pre-coated with an Mouse Cyclin-dependent kinase 12, Cdk12 antibody specific to Mouse Cdk12 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cdk12 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cdk12 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cdk12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验CDK Pathway: Cyclin-Dependent Kinases (cdks) and Cyclin-Dependent Kinase Inhibitors
In elucidating a novel cell-cycle pathway or mode of regulation, it will first be necessary to establish that the cell is arrested in a particular stage of the cell cycle following arrest-inducing conditions. This is achieved by staining
Cyclin-Dependent Kinase 5 (Cdk5): Preparation and Measurement of Kinase Activity
Cyclin-dependent kinase 5 (Cdk5) is a versatile protein kinase that plays a role in a variety of neuronal activities including neuronal migration during brain development, synaptic activities in mature neurons, and neuronal
Assays for Cyclin-Dependent Kinase Inhibitors
Cyclin-dependent kinases (CDK) are serine/threonine kinases that regulate cell cycle control and progression (1 ,2 ). A CDK holoenzyme complex is active if associated with its cyclin partner and if the complex is phosphorylated at specific
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