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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Cyclin-dependent kinase 4, Cdk4含量
- 适应物种:
小鼠
- 标记物:
Cyclin-dependent kinase 4, Cdk4
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Cyclin-dependent kinase 4, Cdk4 ELISA试剂盒
Product Name: Mouse Cyclin-dependent kinase 4, Cdk4 ELISA试剂盒
Product Name: Mouse Cyclin-dependent kinase 4, Cdk4 ELISA试剂盒
Gene Name: Mouse Cyclin-dependent kinase 4, Cdk4
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Cyclin-dependent kinase 4, Cdk4
Alternative Name: Mouse Cyclin-dependent kinase 4, Cdk4
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Cyclin-dependent kinase 4, Cdk4 ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Cyclin-dependent kinase 4, Cdk4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Cyclin-dependent kinase 4, Cdk4 ELISA试剂盒 has been pre-coated with an Mouse Cyclin-dependent kinase 4, Cdk4 antibody specific to Mouse Cyclin-dependent kinase 4, Cdk4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cyclin-dependent kinase 4, Cdk4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cyclin-dependent kinase 4, Cdk4 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cyclin-dependent kinase 4, Cdk4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Assays for Cyclin-Dependent Kinase Inhibitors
of the Retinoblastoma gene product (Rb) by CDK4 and/or CDK6 (G1/S checkpoint), leading to the release of bound E2F from Rb and allowing the transcription of genes by “free E2F” necessary for S phase progression. During late G1 phase, CDK2/cyclin E complex phosphorylates
Translocation Assays of Protein Kinase C Activation
The protein kinase C (PKC) family members include at least 11 different isoforms that, based on their different requirements for activation, have been divided into three subfamilies, the Ca2+ -dependent (cPKCα, (βI , βII
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
of substrates by protein kinases regulates a myriad of cellular processes, ranging from proliferation and migration to autophagy, senescence, and apoptosis. Kinase substrate selectivity is largely dependent on the amino acid sequence surrounding
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