Protein arginine N-methyltrans
ferase 1, Prmt1 ELISA KIT/ Mouse Protein arginine N-methyltransferase 1, Prmt1 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-24207m
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Protein arginine N-methyltransferase 1, Prmt1
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Protein arginine N- methyltransferase 1, Prmt1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Search name
Mouse Prmt1 ELISA KIT ,Mouse HMT2 ELISA KIT, Mouse IR1B ELISA KIT ,Mouse Mrmt1 ELISA KIT ,Mouse ANM1 ELISA KIT ,Mouse HCP1 ELISA KIT ,Mouse HRMT1L2 ELISA KIT ,Mouse IR1B4 ELISA KIT ,Mouse HMT1 (hnRNP methyltransferase ELISA KIT ,Mouse S. cerevisiae)-like 2 ELISA KIT ,Mouse heterogeneous nuclear ribonucleoprotein methyltransferase 1-like 2 ELISA KIT ,Mouse interferon receptor 1-bound protein 4 ELISA KIT ,Mouse protein arginine methyltransferase 1 ELISA KIT ,Mouse histone-arginine N-methyltransferase PRMT1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Protein arginine N-methyltransferase 1, Prmt1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Protein arginine N-methyltransferase 1,Prmt1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Mouse Protein arginine N-methyltransferase 1,Prmt1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Protein arginine N-methyltransferase 1,Prmt1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse Protein arginine N-methyltransferase 1,Prmt1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual
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文献和实验Typical modification sites: RGG box or RXR sequence motifs R-arginine, G-glycine, X-any aminoacid. Enzymes catalysing protein arginine methylation: PRMT1 from rat and the human homologue HRMT1L2, human PRMT2 (HRMT1L1), rat and human PRMT
DamIP: Using Mutant DNA Adenine Methyltransferase to Study DNA‐Protein Interactions In Vivo
for studying DNA?protein interaction in vivo. A mutant form of DNA adenine methyltransferase (DamK9A) from E. coli is fused to the protein of interest and expressed. The fusion protein will bind to target binding sites and introduce N 6 ?adenine methylation
Functional Assays on High‐Content Protein Microarrays
Protein arginine methyltransferase (PRMT1; Sigma, cat. no. P0083‐25UG) S ‐[methyl‐3 H]adenosyl‐L‐methionine ([3 H]SAM; Perkin Elmer, cat. no. NET155V250UC
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