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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Small cell adhesion glycoprotein, SMAGP
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Small cell adhesion glycoprotein, SMAGP ELISA Kit
Product Name:Mouse Small cell adhesion glycoprotein, SMAGP ELISA Kit
Packing:96T
Catalog No.:ELI-29843m
Gene Name:SMAGP
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Mouse Small cell adhesion glycoprotein
Alternative Name:SMAGP,Mouse Small cell adhesion glycoprotein
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Small cell adhesion glycoprotein, SMAGP ELISA Kit allows for the in vitro quantitative determination ofMouse Small cell adhesion glycoprotein concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Small cell adhesion glycoprotein, SMAGP ELISA Kit has been pre-coated with an SMAGP antibody specific to Mouse Small cell adhesion glycoprotein .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Small cell adhesion glycoprotein and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Small cell adhesion glycoprotein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Small cell adhesion glycoprotein in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Heterotypic Cell Adhesion Assay for the Study of Cell Adhesion Inhibition
assay for studying cell adhesion inhibition. The method relies on the CD2 expression on the surface of Jurkat cells and the CD58 expression on the surface of Caco-2 cells, which were confirmed by flow cytometry and ELISA studies respectively
Specific gene silencing using small hairpin RNA (shRNA) constructs offer researchers the possibility to study the influence of a single protein in the metastatic process. The role of the cellular adhesion molecule CEACAM1 on tumour formation
Sacrifice mouse by cervical dislocation. Immerse mouse in 70 Ethanol. Remove spleen ( on the left side ) and transfer into a small petri dish which contains IMDM at room temp. Clean fat from the spleen and transfer the spleen
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