Myosin-binding protein H, Mybp
h ELISA KIT/ Mouse Myosin-binding protein H, Mybph ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-22847m
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Myosin-binding protein H, Mybph
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Myosin- binding protein H, Mybph ELISA KIT
Product Name:Mouse Myosin- binding protein H, Mybph ELISA KIT
Packing:96T
Catalog No.:ELI-22847m
Gene Name:Mouse Mybph
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Mybph
Alternative Name:Mouse Myosin-binding protein H, Mybph
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Myosin- binding protein H, Mybph ELISA KIT allows for the in vitro quantitative determination of Mouse Mybph concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Myosin- binding protein H, Mybph ELISA KIT has been pre-coated with an Mouse Myosin-binding protein H, Mybph antibody specific to Mouse Mybph .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Mybph and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Mybph , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Mybph in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
with non-immune reactivity for immunoglobulin G. J. Immunol. 111:1401-1406. Richman, D. D., Cleveland, P. H., Oxman, M. N., and Johnson, K. M. 1982. The binding of staphylococcl protein A by the sera of different animal species. J. Immunol. 128
Protein purification: skeletal muscle myosin
myosin at 30 ml/h; wash with 350 ml solution E; elute with linear gradient of 0-0.5 M KCl in solution E (2 x 1 liter); collect 10 ml fractions; pool myosin fractions (>0.1 M KCl); concentrate by overnight dialysis against solution F; centrifuge at 25,000
Oligonucleotide-Targeted RNase H Protection Analysis of RNA-Protein Complexes
, oligonucleotide-targeted RNase H cleavage has been used in various systems to disrupt RNPs for functional studies and in protection assays to map RNP protein-binding domains. Examples include in vitro studies that established snRNA sequences essential
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