Nucleolar protein 4, Nol4 ELIS

Mouse Nucleolar protein 4, Nol4 ELISA KIT

Product Name:Mouse Nucleolar protein 4, Nol4 ELISA KIT
Packing:96T

Catalog No.:ELI-22275m

Gene Name:Mouse Nol4

Detect Range:31.2-2000pg/ml

Sensitivity:18.75pg/ml

Target Protein Name:Mouse Nol4

Alternative Name:Mouse Nucleolar protein 4, Nol4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Nucleolar protein 4, Nol4 ELISA KIT allows for the in vitro quantitative determination of Mouse Nol4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Nucleolar protein 4, Nol4 ELISA KIT has been pre-coated with an Mouse Nucleolar protein 4, Nol4 antibody specific to Mouse Nol4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Nol4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Nol4 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Nol4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

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