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- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
THUMP domain-containing protein 3, Thumpd3
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse THUMPD3 ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Mouse THUMPD3 ELISA KIT ,Mouse THUMP domain-containing protein 3 ELISA KIT ,Mouse THUMP domain containing 3 ELISA KIT
Intended use
Mouse THUMPD3 ELISA kit allows for the in vitro quantitative determination of Mouse THUMPD3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
Mouse THUMPD3 ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for THUMPD3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain THUMPD3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of THUMPD3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse THUMPD3 ELISA kit Range 0.15-10ng/ml
Order THUMPD3 ELISA kit by
Human THUMPD3 ELISA kit
Bovine THUMPD3 ELISA kit
Mouse THUMPD3 ELISA kit
:
:
: 3128136151
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文献和实验Eccles:Protein Lysates from Tissue
Cell Lysis Buffer containing 1x Complete protease inhibitor (for embryonic mouse kidney 100uL is suitable) Crush tissue using pestle until tissue is completely pulverised taking care to keep it cold Incubate on ice for 5-10min
purification of protein complexes, in combination with in vivo biotinylation of critical transcription factors, has contributed to the analysis of the pluripotent state in mouse embryonic stem (ES) cells and made it possible to construct a protein?protein
Transgenic Mouse and Transgenic Rat Outline
in the mouse. There are several ways to achieve this. You may use a reporter gene such as a fluorescent protein that you can visualize directly. You may use a reporter gene that provides for enzymatic amplification of the signal such as beta
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