E3 UFM1-protein ligase 1, Ufl1
ELISA KIT/ Mouse E3 UFM1-protein ligase 1, Ufl1 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-22236m
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
E3 UFM1-protein ligase 1, Ufl1
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse E3 UFM1- protein ligase 1, Ufl1 ELISA KIT
Product Name:Mouse E3 UFM1- protein ligase 1, Ufl1 ELISA KIT
Packing:96T
Catalog No.:ELI-22236m
Gene Name:Mouse Ufl1
Detect Range:15.62-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Ufl1
Alternative Name:Mouse E3 UFM1-protein ligase 1, Ufl1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse E3 UFM1- protein ligase 1, Ufl1 ELISA KIT allows for the in vitro quantitative determination of Mouse Ufl1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse E3 UFM1- protein ligase 1, Ufl1 ELISA KIT has been pre-coated with an Mouse E3 UFM1-protein ligase 1, Ufl1 antibody specific to Mouse Ufl1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ufl1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ufl1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ufl1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Purification and Characterization of Legionella U-Box-Type E3 Ubiquitin Ligase
Bacterial virulence proteins often mimic host eukaryotic proteins to modify or disturb host cellular �pathways. Increasing lines of evidence show that many bacterial effector proteins have E3 ubiquitin ligase activity. The effector protein
In Vitro Protein Ubiquitination Assay
Ubiquitination is one of the most important posttranslational modifications in all eukaryote organisms. Ubiquitin-activating enzyme (E1), ubiquitin-conjugating enzyme (E2), and ubiquitin ligase (E3) are the three key enzymes in this process
Functional Assays on High‐Content Protein Microarrays
?protein interactions, protein?DNA interactions, protein?phospholipid interactions, small?molecule targets, substrates of protein kinases, substrates of ubiquitin E3 ligases, and substrates of methyltransferase. This article provides a detailed illustration
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