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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Histone H2B subacrosomal variant, Subh2bv含量
- 适应物种:
小鼠
- 标记物:
Histone H2B subacrosomal variant, Subh2bv
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Histone H2B subacrosomal variant, Subh2bv ELISA KIT
Product Name:Mouse Histone H2B subacrosomal variant, Subh2bv ELISA KIT
Packing:96T
Catalog No.:ELI-08802m
Gene Name:Mouse Subh2bv
Detect Range:15.6-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Subh2bv
Alternative Name:Mouse Histone H2B subacrosomal variant, Subh2bv
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Histone H2B subacrosomal variant, Subh2bv ELISA KIT allows for the in vitro quantitative determination of Mouse Subh2bv concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Histone H2B subacrosomal variant, Subh2bv ELISA KIT has been pre-coated with an Mouse Histone H2B subacrosomal variant, Subh2bv antibody specific to Mouse Subh2bv .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Subh2bv and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Subh2bv , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Subh2bv in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
, H+ transporting, lysosomal interacting protein 1 (ATP6IP1), mRNA 1089 NM_003526 Homo sapiens H2B histone family, member L (H2BFL), mRNA 2232 NM_004718 Homo sapiens cytochrome c oxidase subunit VIIa polypeptide 2 like (COX7A2L), nuclear gene
、双甲基化或三甲基化有关。组蛋白修饰最基本的作用是调控基因表达。例如组蛋白甲基化多导致基因沉默,去甲基化则相反;乙酰化一般是转录激活,去乙酰化则相反。当然,也可在此基础上产生复杂的生物学效应。例如组蛋白去乙酰化酶 HDAC 可影响免疫系统;H3K4me3、H3K9me2 能够调控记忆的形成, 而且 H3K 甲基化与 X 染色体失活、基因组印记和异染色质形成有关;H3 乙酰化通过多种机制调控以来 ATP 的染色质重塑 [12],并参与炎症反应;H2A、H2B 泛素化则与 DNA 损害反应有关;而 H3
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