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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Uridine-cytidine kinase 2, Uck2
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Uridine- cytidine kinase 2, UCK2 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
UCK2,TSA903; UMPK; cytidine monophosphokinase 2; testis-specific protein TSA903; uridine kinase; uridine monophosphate kinase; uridine monophosphokinase 2; uridine-cytidine kinase 2
Search name
Mouse UCK2 ELISA KIT ,Mouse TSA903 ELISA KIT ,Mouse UMPK ELISA KIT ,Mouse cytidine monophosphokinase 2 ELISA KIT ,Mouse testis-specific protein TSA903 ELISA KIT ,Mouse uridine kinase ELISA KIT ,Mouse uridine monophosphate kinase ELISA KIT ,Mouse uridine monophosphokinase 2 ELISA KIT ,Mouse uridine-cytidine kinase 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Uridine- cytidine kinase 2, UCK2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Uridine-cytidine kinase 2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Uridine-cytidine kinase 2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Uridine-cytidine kinase 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Uridine-cytidine kinase 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product
Delivery time 4-6month
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文献和实验【申请上传】Current Protocols in Immunology
and Human Interleukin 9 Unit 6.14 Measurement of Mouse and Human Interleukin 10 Unit 6.15 Measurement of Human Interleukin 11 Unit 6.16 Measurement of Human and Mouse Interleukin-12 Unit 6.17 Measurement of Human and Murine Stem Cell Factor (c-kit Ligand
isolation kit, Qiagen), pooled mRNA for each treatment group. As well as GAPDH, factor VII, glucose-6-phosphatase and VEGF mRNAs were also used for normalization. ELISA was used to quantify the reduction of apoB-100 protein levels in mouse plasma
place through one of two pathways. In one pathway, long dsRNAs activate a protein kinase, PKR. Activated PKR, in turn phoshorylates and inactivates the translation initiation factor, eIF2a, leading to repression of translation. (39) In the other pathway
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