Pituitary adenylate cyclase-ac
tivating polypeptide type I/ Mouse Pituitary adenylate cyclase-activating polypeptide type I- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-21971m
- 2026年01月01日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Pituitary adenylate cyclase-activating polypeptide type I
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Pituitary adenylate cyclase- activating polypeptide type I receptor, ADCYAP1R1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
ADCYAP1R1,PACAP-R1; PAC1; PAC1R; PACAPR; PACAPRI; PACAP-R-1,PACAP receptor 1; PACAP type I receptor; pituitary adenylate cyclase activating polypeptide 1 receptor type I Hiphop; pituitary adenylate cyclase-activating polypeptide type I receptor; adenylate cyclase activating polypeptide 1 (pituitary) receptor type I
Search name
Mouse ADCYAP1R1 ELISA KIT ,Mouse PACAP-R1 ELISA KIT ,Mouse PAC1 ELISA KIT ,Mouse PAC1R ELISA KIT ,Mouse PACAPR ELISA KIT ,Mouse PACAPRI ELISA KIT ,Mouse PACAP-R-1 ELISA KIT ,Mouse PACAP receptor 1 ELISA KIT ,Mouse PACAP type I receptor ELISA KIT ,Mouse pituitary adenylate cyclase activating polypeptide 1 receptor type I Hiphop ELISA KIT ,Mouse pituitary adenylate cyclase-activating polypeptide type I receptor ELISA KIT ,Mouse adenylate cyclase activating polypeptide 1 (pituitary) receptor type I ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse Pituitary adenylate cyclase-activating polypeptide type i receptor, PACAP type I receptor concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to PACAP type I receptor. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PACAP type I receptor and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PACAP type I receptor, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PACAP type I receptor in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验相关专题 细胞凋亡专题 Vasoactive intestinal peptide (VIP) and the structurally related pituitary adenylate cyclase-activating polypeptide (PACAP), two neuropeptides present in the lymphoid microenvironment, elicit
. To address this question, we used the mouse pituitary AtT20 cell line as a model. We compared the proteomic pattern of control cells to that of cells overexpressing proSAAS, a PC1-specific inhibitor. The comparison was conducted using two-dimensional (2D) gel
Activating the NLRP3 Inflammasome Using the Amyloidogenic Peptide IAPP
an inflammasome complex that ultimately cleaves pro-IL-1β and pro-IL-18 into their mature, secreted forms. Here we describe a protocol by which one type of amyloidogenic peptide, islet amyloid polypeptide (IAPP, otherwise known as amylin) can be prepared and used
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