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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Splicing factor 3A subunit 3, Sf3a3
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Splicing factor 3A subunit 3, Sf3a3 ELISA KIT
Product Name:Mouse Splicing factor 3A subunit 3, Sf3a3 ELISA KIT
Packing:96T
Catalog No.:ELI-42293m
Gene Name:Mouse Sf3a3
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Sf3a3
Alternative Name:Mouse Splicing factor 3A subunit 3, Sf3a3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Splicing factor 3A subunit 3, Sf3a3 ELISA KIT allows for the in vitro quantitative determination of Mouse Sf3a3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Splicing factor 3A subunit 3, Sf3a3 ELISA KIT has been pre-coated with an Mouse Splicing factor 3A subunit 3, Sf3a3 antibody specific to Mouse Sf3a3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Sf3a3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Sf3a3 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Sf3a3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
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v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
may be due to growth factor inhibition being mediatedthrough N-terminal phosphorylation of GSK3, resulting ininhibition of primed substrates, and Wnt inhibition focussing ona specific GSK3-containing complex. In addition, growth factorsregulate both GSK3a and GSK
was unresponsive to the transcription factors (Fig. 4D). The sequence 5'-GGAA-3' (ETS-binding site-2, EBS2) is conserved between mouse and the human promoters, and in the mouse it interacts with the ETS-1 protein, a transcription factor of the ETS family expressed
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