Embryonic-type alkaline phosph
atase, Akp5 ELISA KIT/ Mouse Embryonic-type alkaline phosphatase, Akp5 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-16525m
- 2026年01月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Embryonic-type alkaline phosphatase, Akp5
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Embryonic- type alkaline phosphatase, Akp5 ELISA KIT
Product Name:Mouse Embryonic- type alkaline phosphatase, Akp5 ELISA KIT
Packing:96T
Catalog No.:ELI-16525m
Gene Name:Mouse Akp5
Detect Range:15.6-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Akp5
Alternative Name:Mouse Embryonic-type alkaline phosphatase, Akp5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Embryonic- type alkaline phosphatase, Akp5 ELISA KIT allows for the in vitro quantitative determination of Mouse Akp5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Embryonic- type alkaline phosphatase, Akp5 ELISA KIT has been pre-coated with an Mouse Embryonic-type alkaline phosphatase, Akp5 antibody specific to Mouse Akp5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Akp5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Akp5 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Akp5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of X-gal can be easier to detect prior to carrying out the X-Phos reaction as background alkaline phosphatase staining can obscure it, particularly in whole mounts. Rinse the tissue very well with PBS prior to PLAP staining as residual ß-gal activity
Large-Scale Expansion of Mouse Embryonic Stem Cells on Microcarriers
A large-scale stirred culture system for the expansion of mouse embryonic stem cells (mESCs) in spinner flasks under serum-free conditions was established using macroporous microcarriers for cell attachment and growth. This type
Culture of Human Embryonic Stem Cells on Human and Mouse Feeder Cells
or mouse feeder cells, including alkaline phosphatase staining, immunostaining for cell-surface markers associated with undifferentiated hES cells, and teratoma formation in mice.
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