• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Culture of Human Embryonic Stem Cells on Human and Mouse Feeder Cells

        互联网

        618
        This chapter describes the methods we use to maintain and expand undifferentiated human embryonic stem (hES) cells on human and mouse feeder cells. All of the available hES cells have been derived and propagated on primary mouse embryonic fibroblasts as feeder cells that have been mitotically inactivated. We found that hES cells can be successfully cultured on selected human feeder cells, such as marrow stromal cells derived from adult bone marrow and breast skin fibroblasts. Detailed protocols to use human and mouse feeder cells are described here, together with our method to split hES cells by trypsin/ethylenediaminetetraacetic acid-mediated dissociation. We also describe methods we use to characterize hES cells expanded on either human or mouse feeder cells, including alkaline phosphatase staining, immunostaining for cell-surface markers associated with undifferentiated hES cells, and teratoma formation in mice.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序