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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
DNA replication licensing factor MCM6, Mcm6
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse DNA replication licensing factor MCM6, Mcm6 ELISA KIT
Product Name:Mouse DNA replication licensing factor MCM6, Mcm6 ELISA KIT
Packing:96T
Catalog No.:ELI-16344m
Gene Name:Mouse Mcm6
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Mcm6
Alternative Name:Mouse DNA replication licensing factor MCM6, Mcm6
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse DNA replication licensing factor MCM6, Mcm6 ELISA KIT allows for the in vitro quantitative determination of Mouse Mcm6 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse DNA replication licensing factor MCM6, Mcm6 ELISA KIT has been pre-coated with an Mouse DNA replication licensing factor MCM6, Mcm6 antibody specific to Mouse Mcm6 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Mcm6 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Mcm6 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Mcm6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验DamIP: Using Mutant DNA Adenine Methyltransferase to Study DNA‐Protein Interactions In Vivo
.10. © 2011 by John Wiley & Sons, Inc. Keywords: DNA adenine methyltransferase; transcription factor binding sites; DamIP; immunoprecipitation; chromatin immunoprecipitation
is the most basic procedure to study the effects of a soluble factor on aNSC proliferation. We also describe a dual replication labeling protocol using two different halogenated thymidine analogs, chloro? and iododeoxyuridine (CldU and IdU), that allows tracking
large combinatorial libraries of artificial transcription factors comprising three or six zinc-finger domains, and selected transcription factor�DNA interactions able to upregulate several genes in human cells. Selected transcription factors
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