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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Pre-mRNA-processing factor 19, Prpf19
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Pre- mRNA- processing factor 19, Prpf19 ELISA KIT
Product Name:Mouse Pre- mRNA- processing factor 19, Prpf19 ELISA KIT
Packing:96T
Catalog No.:ELI-15673m
Gene Name:Mouse Prpf19
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Prpf19
Alternative Name:Mouse Pre-mRNA-processing factor 19, Prpf19
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Pre- mRNA- processing factor 19, Prpf19 ELISA KIT allows for the in vitro quantitative determination of Mouse Prpf19 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Pre- mRNA- processing factor 19, Prpf19 ELISA KIT has been pre-coated with an Mouse Pre-mRNA-processing factor 19, Prpf19 antibody specific to Mouse Prpf19 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Prpf19 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Prpf19 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Prpf19 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验should be analyzed. Protein levels can be monitored by Western blot, immunofluorescence, ELISA or other means. (Protein can be isolated using Ambion's PARIS™ Kit.) For quantitating mRNA levels, however, qRT-PCR is again the preferred choice.An example
in the promoters of both mouse and human BIC genes. Putative NF-{kappa}B sites are present in the upstream region of the BIC promoter, and previous studies on the increased production of miR-155 in B cells have implicated NF-{kappa}B (15). This transcription factor
-designed using the Cenix algorithm from Ambion. Designs are currently available for >98% of the human, mouse, and rat genes in the RefSeq database. See the Pre-designed siRNA Catalog Page for more information. In addition, Ambion offers Silencer Validated
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