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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
cGMP-inhibited 3',5'-cyclic phosphodiesterase A, Pde3a E
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse PDE3A ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Mouse PDE3A ELISA KIT ,Mouse CGI-PDE ELISA KIT ,Mouse CGI-PDE A ELISA KIT ,Mouse CGI-PDE-A ELISA KIT ,Mouse HTNB ELISA KIT ,Mouse cGMP-inhibited 3' ELISA KIT ,Mouse 5'-cyclic phosphodiesterase A ELISA KIT ,Mouse cAMP phosphodiesterase ELISA KIT ,Mouse myocardial cGMP-inhibited ELISA KIT ,Mouse cyclic GMP-inhibited phosphodiesterase A ELISA KIT ,Mouse phosphodiesterase 3A ELISA KIT ,Mouse cGMP-inhibited ELISA KIT ,Mouse phosphodiesterase 3A ELISA KIT
Intended use
Mouse PDE3A ELISA kit allows for the in vitro quantitative determination of Mouse PDE3A concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
Mouse PDE3A ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PDE3A and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PDE3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PDE3A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse PDE3A ELISA kit Range 15.6-1000pg/ml
Order PDE3A ELISA kit by
Human PDE3A ELISA kit
Mouse PDE3A ELISA kit
Rat PDE3A ELISA kit
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文献和实验Mouse Strains and Genetic Nomenclature
Bishop, C.E., Boursot, P., Baron, B., Bonhomme, F., and Hatat, D. 1985. Most classical Mus musculus domesticus laboratory mouse strains carry a Mus musculus musculus Y chromosome. Nature 315:70‐72
or 4°C; Wash 3 times in 1X PBS for 5 minutes each; Permeabilize with freshly made 1X PBS/0.5% Triton X-100 (containing the RNAse-inhibitor, 2mM Vanadyl Ribonucleoside Complex, in case of a subsequent RNA-FISH) on ice for 3.5-5 minutes
Characterization and Validation of Cre‐Driver Mouse Lines
. Birling, M.C., Gofflot, F., and Warot, X. 2009. Site‐specific recombinases for manipulation of the mouse genome. Methods Mol. Biol. 561:245‐263.
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