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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Mitogen-activated protein kinase kinase kinase kinase 1,
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Mitogen-activated protein kinase kinase kinase kinase 1, MAP4K1 ELISA Kit (CUSTOM)
96 Tests
Operating instructions
FOR RESEARCH USE ONLY!
Alternative name
Mouse Map4k1 ELISA KIT ,Mouse hematopoietic progenitor kinase 1 ELISA KIT ,Mouse HPK1 ELISA KIT ,Mouse MAPK/ERK kinase kinase kinase 1 ELISA KIT ,Mouse MEK kinase kinase 1 ELISA KIT ,Mouse MEKKK 1 ELISA KIT ,Mouse mitogen-activated protein kinase kinase kinase kinase 1 ELISA KIT
Intended use
Mouse MAP4K1 ELISA kit allows for the in vitro quantitative determination of Mouse Mitogen-activated protein kinase kinase kinase kinase 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
Mouse MAP4K1 ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MAP4K1, During the reaction, MAP4K1 in the sample or standard competes with a fixed amount of biotin-labeled MAP4K1 for sites on a pre-coated Monoclonal antibody specific to MAP4K1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MAP4K1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product Min.Order 5kits
Delivery time 4-6month
Order Mouse MAP4K1 ELISA kit by email:
:
:
: 3128136151
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文献和实验Mitogen-Activated Protein Kinase Assays
-activated protein kinases (MAPKs) and their activation kinase cascades, which transduce signals from the plasma membrane to the cytosol and nucleus, are an integral part of signaling pathways involved in many cellular responses. PMN express several members
Analysis of Mitogen-Activated Protein Kinase Activation
signal-regulated kinases, JNK, and p38MAPK also play a significant role in the regulation of mast cell biological function. This chapter will detail recent advances in determining mitogen-activated protein kinase activation in single cells. These methods
Mitogen-Activated Protein Kinase Pathways in Interferon Signaling
There is recent evidence that in addition to the classic JAK/STAT pathways, mitogen-activated proteins (MAP) kinase pathways play important roles in Type I interferon signaling and the generation of interferon responses. In particular
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