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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Nik-related protein kinase, Nrk
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Nik- related protein kinase, Nrk ELISA KIT
Product Name:Mouse Nik- related protein kinase, Nrk ELISA KIT
Packing:96T
Catalog No.:ELI-13751m
Gene Name:Mouse Nrk
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Nrk
Alternative Name:Mouse Nik-related protein kinase, Nrk
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Nik- related protein kinase, Nrk ELISA KIT allows for the in vitro quantitative determination of Mouse Nrk concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Nik- related protein kinase, Nrk ELISA KIT has been pre-coated with an Mouse Nik-related protein kinase, Nrk antibody specific to Mouse Nrk .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Nrk and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Nrk , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Nrk in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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The progress made in understanding the molecular basis of mammalian cell transformation has led to the unifying concept of growth regulation and its disorders in cancer cells. Today it is well recognized that many products of “cancer genes
Visualization of Protein Kinase Activities in Living Cells
organisms ranging from Caenorhabditis elegans to mouse, permitting visualization of spatio-temporal regulation of kinase activities and systemic understanding of the signaling networks in living animals.
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