SH2 domain-containing adapter

Mouse SH2 domain- containing adapter protein B, Shb ELISA KIT

Product Name:Mouse SH2 domain- containing adapter protein B, Shb ELISA KIT
Packing:96T

Catalog No.:ELI-13521m

Gene Name:Mouse Shb

Detect Range:31.25-2000pg/ml

Sensitivity:18.75pg/ml

Target Protein Name:Mouse Shb

Alternative Name:Mouse SH2 domain-containing adapter protein B, Shb
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse SH2 domain- containing adapter protein B, Shb ELISA KIT allows for the in vitro quantitative determination of Mouse Shb concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse SH2 domain- containing adapter protein B, Shb ELISA KIT has been pre-coated with an Mouse SH2 domain-containing adapter protein B, Shb antibody specific to Mouse Shb .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Shb and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Shb , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Shb in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

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