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- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白B-cell lymphoma含量
- 适应物种:
小鼠
- 标记物:
B-cell lymphoma
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse B- cell lymphoma/leukemia 11A, Bcl11a ELISA KIT
Product Name:Mouse B- cell lymphoma/leukemia 11A, Bcl11a ELISA KIT
Packing:96T
Catalog No.:ELI-11446m
Gene Name:Mouse Bcl11a
Detect Range:15.6-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Bcl11a
Alternative Name:Mouse B-cell lymphoma/leukemia 11A, Bcl11a
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse B- cell lymphoma/leukemia 11A, Bcl11a ELISA KIT allows for the in vitro quantitative determination of Mouse Bcl11a concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse B- cell lymphoma/leukemia 11A, Bcl11a ELISA KIT has been pre-coated with an Mouse B-cell lymphoma/leukemia 11A, Bcl11a antibody specific to Mouse Bcl11a .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Bcl11a and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Bcl11a , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Bcl11a in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
in PCR walking. Nucleic Acids Res 23, 1644–5. 27. Yin, B., Delwel, R., Valk, P. J., Wallace, M. R.,Loh, M. L., Shannon, K. M., and Largaespada, D. A. (2009) A retroviral mutagenesis screen reveals strong cooperation between Bcl11a overexpression
Construction of Gene-Targeting Vectors by Recombineering
and made recombineering competent by pSim18 plasmid transformation. Exon 4, which encodes the main functional domains of the Bcl11a protein, is selected to be the deleted region and would be flanked by lox P sites in the conditional allele. (B ) The Bsd cassette flanked
CD66a/CD66b/CD66c/CD66d/CD66e/CD66f/CD67分子
ELISA KIT & ANTIBODY OF USCNLIFE
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