Beta-defensin 12, Defb12 ELISA

Mouse Beta- defensin 12, Defb12 ELISA KIT

Product Name:Mouse Beta- defensin 12, Defb12 ELISA KIT
Packing:96T

Catalog No.:ELI-47011m

Gene Name:Mouse Defb12

Detect Range:15.62-1000pg/ml

Sensitivity:9.375pg/ml

Target Protein Name:Mouse Defb12

Alternative Name:Mouse Beta-defensin 12, Defb12
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Beta- defensin 12, Defb12 ELISA KIT allows for the in vitro quantitative determination of Mouse Defb12 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Beta- defensin 12, Defb12 ELISA KIT has been pre-coated with an Mouse Beta-defensin 12, Defb12 antibody specific to Mouse Defb12 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Defb12 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Defb12 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Defb12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!