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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Cytochrome b reductase 1, Cybrd1含量
- 适应物种:
小鼠
- 标记物:
Cytochrome b reductase 1, Cybrd1
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Cytochrome b reductase 1, Cybrd1 ELISA KIT
Product Name:Mouse Cytochrome b reductase 1, Cybrd1 ELISA KIT
Packing:96T
Catalog No.:ELI-09013m
Gene Name:Mouse Cybrd1
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Cybrd1
Alternative Name:Mouse Cytochrome b reductase 1, Cybrd1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Cytochrome b reductase 1, Cybrd1 ELISA KIT allows for the in vitro quantitative determination of Mouse Cybrd1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Cytochrome b reductase 1, Cybrd1 ELISA KIT has been pre-coated with an Mouse Cytochrome b reductase 1, Cybrd1 antibody specific to Mouse Cybrd1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cybrd1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cybrd1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cybrd1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验细胞色素还原酶(系统) cytochrome reductase(system)
还原细胞色素 c的氧化还原酶系统的一种。有许多报告指出,线粒体的酶系统构成呼吸链的一部分,可用 NADH或琥珀酸还原细胞色素 c。用胆酸处理线粒体可获得。认为是在 NADH-泛醌,琥珀酸 -泛醌,泛醌-细胞色素 c之间发生由三个颗粒组成的电子传递,含有细胞色素 c、细胞色素 b、醌、非血红素铁和磷脂等。以前所了解的 NAD-细胞色素 c还原酶可以看成是人工提纯处理的。
Cytochrome P450 Reconstitution Systems
adenine dinucleotide phosphate-cytochrome P450 reductase in the membranes of phospholipid vesicles. However, our studies have suggested that the conditions for reconstitution of activities vary depending on the P450 enzymes used. For example
Isolation and Purification of Constitutive Forms of Microsomal Cytochrome P450
, deoxycholate (1 ), allowing the monooxygenase system to be separated into three components, a reductase, a lipid-containing fraction and a cytochrome P450-containing fraction. Diethylammoethyl (DEAE)-chromatography was used in subsequent studies to purify
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