Homeobox protein Nkx-2.2, Nkx2
-2 ELISA KIT/ Mouse Homeobox protein Nkx-2.2, Nkx2-2 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-23178m
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Homeobox protein Nkx-2.2, Nkx2-2
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Homeobox protein Nkx- 2.2, Nkx2-2 ELISA KIT
Product Name:Mouse Homeobox protein Nkx- 2.2, Nkx2-2 ELISA KIT
Packing:96T
Catalog No.:ELI-23178m
Gene Name:Mouse Nkx2-2
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Nkx2-2
Alternative Name:Mouse Homeobox protein Nkx-2.2, Nkx2-2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Homeobox protein Nkx- 2.2, Nkx2-2 ELISA KIT allows for the in vitro quantitative determination of Mouse Nkx2-2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Homeobox protein Nkx- 2.2, Nkx2-2 ELISA KIT has been pre-coated with an Mouse Homeobox protein Nkx-2.2, Nkx2-2 antibody specific to Mouse Nkx2-2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Nkx2-2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Nkx2-2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Nkx2-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Prediction of Protein‐Protein Interaction Networks
Figure 8.2.3 One method of gene fusion. Individual proteins, A and B, from one genome can often be found as a single fused protein, C, in another genome. The finding of such a fused protein suggests
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2
Protein A Purification of Antibody
fractions until base line is achieved.Elute Ab with 5 bed volumes of Elution Buffer. Collect 1 ml fractions into 50 ml 1M Tris, until base line is achieved.Take 50 ml aliquots of each fraction for ELISA.Pool fractions containing Ab. Dialyze against 2 x 4L
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