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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Serine protease inhibitor A3G, Serpina3g含量
- 适应物种:
小鼠
- 标记物:
Serine protease inhibitor A3G, Serpina3g
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Serine protease inhibitor A3G, Serpina3g ELISA KIT
Product Name:Mouse Serine protease inhibitor A3G, Serpina3g ELISA KIT
Packing:96T
Catalog No.:ELI-07625m
Gene Name:Mouse Serpina3g
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Serpina3g
Alternative Name:Mouse Serine protease inhibitor A3G, Serpina3g
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Serine protease inhibitor A3G, Serpina3g ELISA KIT allows for the in vitro quantitative determination of Mouse Serpina3g concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Serine protease inhibitor A3G, Serpina3g ELISA KIT has been pre-coated with an Mouse Serine protease inhibitor A3G, Serpina3g antibody specific to Mouse Serpina3g .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Serpina3g and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Serpina3g , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Serpina3g in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验) or early death. The serine protease uPA, in concert with its inhibitor PAI-1, promotes tumor cell adhesion, migration, and proliferation, as well as extracellular matrix degradation and, thus, facilitates tumor cell invasion and metastasis. The various
Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer
(phenylmethanesulphonylfluoride, a serine protease inhibitor) Sodium orthovanadate (inhibitor of protein tyrosine phosphatases, alkaline phosphatases and a number of ATPases, most likely acting as a phosphate analogue, the VO43- ion binds irreversibly to the active sites
of pericellular proteases; among those, the serine protease, urokinase-type plasminogen activator (uPA), its inhibitor PAI-1, and its cellular receptor uPA-R (CD87). Apart from its proteolytic functions, the uPA system is endowed with properties affecting
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