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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Nuclear cap-binding protein subunit 2, Ncbp2
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Nuclear cap- binding protein subunit 2, Ncbp2 ELISA KIT
Product Name:Mouse Nuclear cap- binding protein subunit 2, Ncbp2 ELISA KIT
Packing:96T
Catalog No.:ELI-44687m
Gene Name:Mouse Ncbp2
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Ncbp2
Alternative Name:Mouse Nuclear cap-binding protein subunit 2, Ncbp2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Nuclear cap- binding protein subunit 2, Ncbp2 ELISA KIT allows for the in vitro quantitative determination of Mouse Ncbp2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Nuclear cap- binding protein subunit 2, Ncbp2 ELISA KIT has been pre-coated with an Mouse Nuclear cap-binding protein subunit 2, Ncbp2 antibody specific to Mouse Ncbp2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ncbp2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ncbp2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ncbp2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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: 3128136151
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文献和实验) ( 2 - 4 ). The proximal promoter, containing the hormone response elements (HREs), and binding sites for nuclear factor 1 (NF1), TATA binding protein (TBP), and octamer transcription factors (OTFs) is encompassed by nucleosomes A and B ( 2 , 5 ). Analysis
. Li, X.Y., Virbasius, A., Zhu, X., and Green, M.R. 1999. Enhancement of TBP binding by activators and general transcription factors. Nature 399:605‐609.
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
stock solution Nutrient-rich fly medium Phosphate-buffered saline (PBS; pH 7.5) Poly-L-lysine solution (0.1% [w/v] in H2 O; Sigma P8920) Sodium phosphate buffer (10 mM, pH 6.8) Triton X-100 VECTASTAIN Elite ABC Kit
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