Semaphorin-3B, Sema3b ELISA KI

Mouse Semaphorin- 3B, Sema3b ELISA KIT

Product Name:Mouse Semaphorin- 3B, Sema3b ELISA KIT
Packing:96T

Catalog No.:ELI-07273m

Gene Name:Mouse Sema3b

Detect Range:78-5000pg/ml

Sensitivity:46.9pg/ml

Target Protein Name:Mouse Sema3b

Alternative Name:Mouse Semaphorin-3B, Sema3b
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Semaphorin- 3B, Sema3b ELISA KIT allows for the in vitro quantitative determination of Mouse Sema3b concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Semaphorin- 3B, Sema3b ELISA KIT has been pre-coated with an Mouse Semaphorin-3B, Sema3b antibody specific to Mouse Sema3b .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Sema3b and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Sema3b , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Sema3b in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

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