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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Cytohesin-interacting protein, Cytip
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Cytohesin- interacting protein, Cytip ELISA KIT
Product Name:Mouse Cytohesin- interacting protein, Cytip ELISA KIT
Packing:96T
Catalog No.:ELI-27040m
Gene Name:Mouse Cytip
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Cytip
Alternative Name:Mouse Cytohesin-interacting protein, Cytip
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Cytohesin- interacting protein, Cytip ELISA KIT allows for the in vitro quantitative determination of Mouse Cytip concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Cytohesin- interacting protein, Cytip ELISA KIT has been pre-coated with an Mouse Cytohesin-interacting protein, Cytip antibody specific to Mouse Cytip .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cytip and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cytip , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cytip in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Phage‐Based Expression Cloning to Identify Interacting Proteins
expression cloning is a simple, rapid, and powerful technique to identify interacting proteins. A protein of interest is expressed as a recombinant fusion protein and labeled with 32 P at an engineered recognition site to facilitate detection. b?gal proteins
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
to establish, by using separate assays, that the interacting regions captured in the 6C assay are truly occupied by the protein of interest in cells. To this end, perform ChIP assays (Tiwari et al. 2008) using antibodies specific for the protein
Prediction of Protein‐Protein Interaction Networks
interacting protein families are first generated along with the 16S ribosomal RNA sequence alignments for the same taxa. (B ) Distance matrices are generated from the alignments (with tree‐of‐life distances subtracted from the distance matrices in the case
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