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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Fumarate hydratase, mitochondrial, Fh含量
- 适应物种:
小鼠
- 标记物:
Fumarate hydratase, mitochondrial, Fh
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Fumarate hydratase, mitochondrial, Fh ELISA试剂盒
Product Name: Mouse Fumarate hydratase, mitochondrial, Fh ELISA试剂盒
Product Name: Mouse Fumarate hydratase, mitochondrial, Fh ELISA试剂盒
Gene Name: Mouse Fumarate hydratase, mitochondrial, Fh
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Fumarate hydratase, mitochondrial, Fh
Alternative Name: Mouse Fumarate hydratase, mitochondrial, Fh
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Fumarate hydratase, mitochondrial, Fh ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Fumarate hydratase, mitochondrial, Fh concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Fumarate hydratase, mitochondrial, Fh ELISA试剂盒 has been pre-coated with an Mouse Fumarate hydratase, mitochondrial, Fh antibody specific to Mouse Fumarate hydratase, mitochondrial, Fh .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Fumarate hydratase, mitochondrial, Fh and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Fumarate hydratase, mitochondrial, Fh , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Fumarate hydratase, mitochondrial, Fh in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Mol Cell:南京医科大学钱旭团队揭示延胡索酸促进肿瘤恶化新机制
的 Fumarate 通过半胱氨酸琥珀酸化修饰 PTEN,抑制 PTEN 生物学活性,进一步促进肿瘤恶性进程。 延胡索酸(Fumarate)是三羧酸循环的重要中间代谢产物,能够通过延胡索酸水合酶(Fumarate Hydratase, FH)生成苹果酸,有研究表明 FH 在多种肿瘤中常发生突变,而 FH 的失活突变直接导致肿瘤细胞内 Fumarate 累积,促进肿瘤的发生发展。已有报道表明 Fumarate 做为 α-酮戊二酸(α-KG)的类似物,能够介导表观遗传重编程,其通过竞争性抑制 α-KG 依赖的组蛋白
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
acyltransferase, alpha) (AGPAT1), mRNA 742 NM_021134 Homo sapiens mitochondrial ribosomal protein L23 (MRPL23), mRNA 771 NM_021974 Homo sapiens polymerase (RNA) II (DNA directed) polypeptide F (POLR2F), mRNA 1169 NM_006808 Homo sapiens protein
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