Calcium-dependent phospholipas
e A2, Pla2g5 ELISA KIT/ Mouse Calcium-dependent phospholipase A2, Pla2g5 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-23294m
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Calcium-dependent phospholipase A2, Pla2g5
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Calcium- dependent phospholipase A2, Pla2g5 ELISA KIT
Product Name:Mouse Calcium- dependent phospholipase A2, Pla2g5 ELISA KIT
Packing:96T
Catalog No.:ELI-23294m
Gene Name:Mouse Pla2g5
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Pla2g5
Alternative Name:Mouse Calcium-dependent phospholipase A2, Pla2g5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Calcium- dependent phospholipase A2, Pla2g5 ELISA KIT allows for the in vitro quantitative determination of Mouse Pla2g5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Calcium- dependent phospholipase A2, Pla2g5 ELISA KIT has been pre-coated with an Mouse Calcium-dependent phospholipase A2, Pla2g5 antibody specific to Mouse Pla2g5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Pla2g5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Pla2g5 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Pla2g5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Assaying Phospholipase A2 Activity
PLA2 α (cPLAα) and for secreted PLA2 s (sPLA2 ) that are suitable for purified enzymes and for measuring activity in crude cell lysates and culture medium. The assay for cPLA2 α involves measuring the calcium-dependent release of radiolabeled sn
Phospholipase A2 Purification and Characterization: A Case Study
We compared here the purification procedures, the pH, the calcium, the bile salts, and the temperature dependencies as well as the catalytic activities on phosphatidylcholine (PC) and phosphatidylethanolamine (PE) of two purified secreted PLA
Calcium Mobilization in Ovarian Cancer Cells in Response to Lysophospholipids
(phospholipase A2 , phospho- lipase D and some isoforms of protein kinase C) associated with the liberation of bioactive lipids such as arachidonic acid (AA) and lysophosphatidic acid (LPA) (1 ,2 ). AA and its metabolites have been implicated in multiple steps
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