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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白B-lymphocyte antigen CD19, Cd19含量
- 适应物种:
小鼠
- 标记物:
B-lymphocyte antigen CD19, Cd19
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse B-lymphocyte antigen CD19, Cd19 ELISA试剂盒
Product Name: Mouse B-lymphocyte antigen CD19, Cd19 ELISA试剂盒
Product Name: Mouse B-lymphocyte antigen CD19, Cd19 ELISA试剂盒
Gene Name: Mouse B-lymphocyte antigen CD19, Cd19
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse B-lymphocyte antigen CD19, Cd19
Alternative Name: Mouse B-lymphocyte antigen CD19, Cd19
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse B-lymphocyte antigen CD19, Cd19 ELISA试剂盒 allows for the in vitro quantitative determination of Mouse B-lymphocyte antigen CD19, Cd19 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse B-lymphocyte antigen CD19, Cd19 ELISA试剂盒 has been pre-coated with an Mouse B-lymphocyte antigen CD19, Cd19 antibody specific to Mouse B-lymphocyte antigen CD19, Cd19 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse B-lymphocyte antigen CD19, Cd19 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse B-lymphocyte antigen CD19, Cd19 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse B-lymphocyte antigen CD19, Cd19 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验thresholds for antigen receptors and other surface receptors on B cells; also regulates B cell development, activation and differentiation CD19 antibody inhibits B cell activation from anti-immunoglobulin Coreceptor with CD21 Earliest B cell
CD19 / CD81 白细胞分化抗原及其功能 CDl9 CDl9分子胞膜外区结构属IgSF,包括2个C2样区,胞质区较长,不同种属间有很高的同源性,含有可发生磷酸化的丝/苏氨酸和酪氨酸残基。CDl9分子分布于除浆细胞外的B细胞谱系发育的各个阶段,也是与B细胞识别、黏附和活化有关的CD分子的结构B细胞的重要标记。 此外CDl9还表达于生发中心的FDC,以及某些急性髓细胞白血病细胞。CDl9是CDl9/CD21/CD81信号复合
Mouse B cell isolation with magnetic beads
实验概要 Isolate B cells from total mouse cells Praparation: 1.Enough MACS; put RPMI into water bath. 2.Put some MACS in 15 ml tube or 1.5 ml tube from cell room
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