Izumo sperm-egg fusion protein
1, Izumo1 ELISA KIT/ Mouse Izumo sperm-egg fusion protein 1, Izumo1 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-05119m
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Izumo sperm-egg fusion protein 1, Izumo1含量
- 适应物种：
小鼠
- 标记物：
Izumo sperm-egg fusion protein 1, Izumo1
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Izumo sperm-egg fusion protein 1, Izumo1 ELISA试剂盒
Product Name: Mouse Izumo sperm-egg fusion protein 1, Izumo1 ELISA试剂盒
Product Name: Mouse Izumo sperm-egg fusion protein 1, Izumo1 ELISA试剂盒
Gene Name: Mouse Izumo sperm-egg fusion protein 1, Izumo1
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Izumo sperm-egg fusion protein 1, Izumo1
Alternative Name: Mouse Izumo sperm-egg fusion protein 1, Izumo1
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Izumo sperm-egg fusion protein 1, Izumo1 ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Izumo sperm-egg fusion protein 1, Izumo1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Izumo sperm-egg fusion protein 1, Izumo1 ELISA试剂盒 has been pre-coated with an Mouse Izumo sperm-egg fusion protein 1, Izumo1 antibody specific to Mouse Izumo sperm-egg fusion protein 1, Izumo1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Izumo sperm-egg fusion protein 1, Izumo1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Izumo sperm-egg fusion protein 1, Izumo1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Izumo sperm-egg fusion protein 1, Izumo1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验mm (100) - ESCO (VWR 9611301) Dextran sulfate - Sigma® 31403 Formamide - Sigma® F 5786 Gelatin 250g - Merck® (VWR 24360233) Glycerol - Sigma® G5516 Mouse X-chromosome paint - Cambio 1187-XMB-02 Paraformaldehyde - RE/PURO 387507 PBS 10X 500ml
were passed through 1-ml nickel affinity columns (B-PER 6×His fusion protein purification kit from Pierce)that had been pre-equilibrated with 7 M GuHCl in TBS (0.15 M NaCl,50 mM Tris.HCl pH 7.5).The columns were washed thrice with 1 ml 7 M GuHCl
Membrane‐Based Yeast Two‐Hybrid System to Detect Protein Interactions
1: Production of Single‐Stranded Carrier DNA from Salmon Sperm Support Protocol 2: Preparation of Membrane Extracts from Yeast Support Protocol 3: Determination of Bait‐Prey Protein Interaction Specificity
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