RUN domain-containing protein
3A, Rundc3a ELISA KIT/ Mouse RUN domain-containing protein 3A, Rundc3a ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-21732m
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
RUN domain-containing protein 3A, Rundc3a
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse RUN domain- containing protein 3A, Rundc3a ELISA KIT
Product Name:Mouse RUN domain- containing protein 3A, Rundc3a ELISA KIT
Packing:96T
Catalog No.:ELI-21732m
Gene Name:Mouse Rundc3a
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Rundc3a
Alternative Name:Mouse RUN domain-containing protein 3A, Rundc3a
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse RUN domain- containing protein 3A, Rundc3a ELISA KIT allows for the in vitro quantitative determination of Mouse Rundc3a concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse RUN domain- containing protein 3A, Rundc3a ELISA KIT has been pre-coated with an Mouse RUN domain-containing protein 3A, Rundc3a antibody specific to Mouse Rundc3a .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Rundc3a and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Rundc3a , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Rundc3a in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Transgenic Mouse and Transgenic Rat Outline
in the mouse. There are several ways to achieve this. You may use a reporter gene such as a fluorescent protein that you can visualize directly. You may use a reporter gene that provides for enzymatic amplification of the signal such as beta
Mapping Protein‐Protein Interactions with Phage‐Displayed Combinatorial Peptide Libraries
Figure 17.4.1 Photograph of a phage ELISA result. Using eight different clones that were isolated with GST‐SrcSH3 domain as the target, 25 µl of culture supernatant were added to pairs of microtiter plate wells containing GST‐SrcSH3 or GST
into the wells of the SDS-PAGE gel. Load an appropriate amount of Trident Blue Prestained Protein Ladder or Trident Prestained Protein Ladder to one or more additional lanes.2. Run the gel in 1X Trident Running Buffer for 1-2 hours at 50-100 V.We recommend
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