Mouse Enoyl-CoA hydratase, mit

Mouse Enoyl- CoA hydratase, mitochondrial, Echs1 ELISA KIT

Product Name:Mouse Enoyl- CoA hydratase, mitochondrial, Echs1 ELISA KIT
Packing:96T

Catalog No.:ELI-03746m

Gene Name:Echs1

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:Mouse Enoyl- CoA hydratase,mitochondrial

Alternative Name:Echs1,Mouse Enoyl- CoA hydratase,mitochondrial
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Enoyl- CoA hydratase, mitochondrial, Echs1 ELISA KIT allows for the in vitro quantitative determination ofMouse Enoyl- CoA hydratase,mitochondrial concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Enoyl- CoA hydratase, mitochondrial, Echs1 ELISA KIT has been pre-coated with an Echs1 antibody specific to Mouse Enoyl- CoA hydratase,mitochondrial .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Enoyl- CoA hydratase,mitochondrial and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Enoyl- CoA hydratase,mitochondrial, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Enoyl- CoA hydratase,mitochondrial in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

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