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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白apoptosis protease activating factor-1含量
- 适应物种:
小鼠
- 标记物:
apoptosis protease activating factor-1
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse apoptosis protease activating factor-1 ELISA试剂盒
Product Name: Mouse apoptosis protease activating factor-1 ELISA试剂盒
Product Name: Mouse apoptosis protease activating factor-1 ELISA试剂盒
Gene Name: Mouse apoptosis protease activating factor-1
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse apoptosis protease activating factor-1
Alternative Name: Mouse apoptosis protease activating factor-1
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse apoptosis protease activating factor-1 ELISA试剂盒 allows for the in vitro quantitative determination of Mouse apoptosis protease activating factor-1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse apoptosis protease activating factor-1 ELISA试剂盒 has been pre-coated with an Mouse apoptosis protease activating factor-1 antibody specific to Mouse apoptosis protease activating factor-1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse apoptosis protease activating factor-1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse apoptosis protease activating factor-1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse apoptosis protease activating factor-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验DNA-Dependent Protein Kinase in Apoptosis
in the generation of an as yet signal that results in the release of cytochrome C from the intermembrane space of mitochondria into the cytoplasm. It is in the cytoplasm that cytochrome C can form a complex with apocaspase 9, apoptotic protease activating factor
T cells and is important in cytotoxic T cell induced apoptosis, activating caspase independent pathways. Once in a cell, Granzyme A activates DNA nicking by the recently identified DNAse NM23-H1, a tumor suppressor gene product whose expression
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
The immunocytochemical procedure described represents an original modification of the method for cell death detection by ELISA. Utilizing this new modified assay we are able to show the presence of apoptotic cells or cells involved in apoptosis in the thymus
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