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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Glycosyltransferase-like protein LARGE1, Large
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Glycosyltransferase- like protein LARGE1, Large ELISA KIT
Product Name:Mouse Glycosyltransferase- like protein LARGE1, Large ELISA KIT
Packing:96T
Catalog No.:ELI-43203m
Gene Name:Mouse Large
Detect Range:15.6-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Large
Alternative Name:Mouse Glycosyltransferase-like protein LARGE1, Large
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Glycosyltransferase- like protein LARGE1, Large ELISA KIT allows for the in vitro quantitative determination of Mouse Large concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Glycosyltransferase- like protein LARGE1, Large ELISA KIT has been pre-coated with an Mouse Glycosyltransferase-like protein LARGE1, Large antibody specific to Mouse Large .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Large and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Large , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Large in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验by a two- or three-step procedure. The method can insert any fragment into small or large episomes, without the need of an antibiotic selection gene. We have humanized the mouse luteinizing hormone receptor gene (Lhcgr ) by inserting a ~55 kb fragment from a BAC clone
of the genes in the genome. It is becoming increasingly accepted that there needs to be a coherent programme of systematic mutation of every protein-coding gene in the mouse genome. The target is therefore to generate approximately 20,000-25,000 gene knockout
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