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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
pCR-Blunt II-TOPO-CASP14
- 库存:
50
- 供应商:
LSMBIO
- 规格:
2ug
pCR-Blunt II-TOPO-CASP14/ pCR-Blunt II-TOPO-CASP14璐ㄧ矑
pCR-Blunt II-TOPO-CASP14 Plasmid
PVT15993 2ug
pCR-Blunt II-TOPO-CASP14 Plasmid Alternative Name
NM_012114.3,ARCI12
pCR-Blunt II-TOPO-CASP14 PlasmidInformation
Promoter
Prokaryotic resistance Amp
Screening marker
Clone strain DH5a
Culture Conditions LB/37 Celsius degree
Forward primer M13R
Reverse primer M13F
pCR-Blunt II-TOPO-CASP14 Plasmid Description
pCR-Blunt II-TOPO-CASP14 Plasmid is a DNA cloning plasmid
NOTE: For research use only!
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文献和实验3’-A overhang to each end of the PCR product. Blunt cloning vectors and directional TOPO® cloning technologies are designed to clone PCR products produced by proofreading polymerases. Successful cloning depends upon using the correct polymerase with your cloning
Random Mutagenesis by Whole-Plasmid PCR Amplification
Mutagenesis is a popular tool used in the analysis of protein structure and function. Polymerase chain reaction (PCR)-based mutagenesis can be used to introduce mutations with the use of the appropriate primer. Although the majority
μL 体系 2× MasterMix 25 μL 上游 Primer ( 10 μM 1-5 μL 下游 Primer ( 10 μM ) 1-5 μL 模板 lamid DNA ~ 50 ng genomic DNA ~ 1000 ng cDNA ~ 500 ng 粗提液 ~ 2 μL 灭菌蒸馏水至 50 μL PCR 扩增实例: 一、plasmid DNA 扩增 1.提取的质粒 DNA 扩增 以pUC19 质粒为模板扩增 100,250,500
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