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- 文献和实验
- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
pOTB7-DHFR
- 库存:
50
- 供应商:
LSMBIO
- 规格:
2ug
pOTB7-DHFR/ pOTB7-DHFR璐ㄧ矑
pOTB7-DHFR
PVT14397 2ug
pOTB7-DHFR Search name
pOTB7-DHFR,pOTB7-DHFR vector,pOTB7-DHFR plasmid,pOTB7-DHFR map
pOTB7-DHFR Information
Bacterial Resistance(s): Chl
Screening:
Growth Strain(s): DH5 alpha
Growth Condition: LB/37 Centigrade
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文献和实验Using the DHFR Heat-Inducible Degron for Protein Inactivation in Schizosaccharomyces pombe
Inactivating a specific protein in vivo can yield important information about its function. One strategy previously developed inSaccharomyces cerevisiae by the Varshavsky group involves fusing a degron, derived from mouse dihydrofolate
This protocol is for Mini (up to 20 µg) preparations of high-copy plasmid DNA from cultures of E. coli . Important notes before starting New users are strongly recommended to read Appendix A (page 21) at the end
Plasmid Profile Typing and Plasmid Fingerprinting
Plasmids are extra-chromosomal molecules of deoxyribonucleic acid (DNA) capable of autonomous replication. Such molecules have been identified in many bacterial genera and usually exist as covalently closed circular (CCC) molecules. Plasmids
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